Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Helix01:16

The DNA Helix

163.6K
Overview
163.6K
The DNA Helix01:07

The DNA Helix

31.9K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
31.9K
The DNA Helix01:16

The DNA Helix

63.3K
63.3K
DNA Base Pairing02:27

DNA Base Pairing

36.4K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
36.4K
DNA Base Pairing02:27

DNA Base Pairing

33.7K
33.7K
DNA as a Genetic Template02:05

DNA as a Genetic Template

29.1K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
29.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Copper-Catalyzed Functionalization of C(sp<sup>2</sup>)-H Bonds with Vinyl Cations: Synthesis of Polycyclic Pyrroles.

Organic letters·2026
Same author

Deep oxidation of single-walled carbon nanotubes toward a versatile platform for direct functionalization.

Nanoscale·2026
Same author

Synthesis and evaluation of new 2-substituted anthra[2,3-b]furan-5,10-diones: tumor cell apoptosis through DNA binding and topoisomerases inhibition.

Bioorganic & medicinal chemistry·2026
Same author

Stereodivergent (3 + 2)-cycloaddition of donor-acceptor cyclopropanes and citral imines catalyzed by Yb(NTf<sub>2</sub>)<sub>3</sub>/PyBOX.

Organic & biomolecular chemistry·2026
Same author

Early Postprandial Response of Skeletal Muscle Phosphoproteome in Type 2 Diabetes Is Comparable to That in Healthy Individuals.

Biochemistry. Biokhimiia·2026
Same author

One-Pot Synthesis of Aminodiperoxides from 1,5-Diketones, Geminal Bishydroperoxides and Ammonium Acetate.

Molecules (Basel, Switzerland)·2025

Related Experiment Video

Updated: Apr 19, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K

Anomeric DNA quadruplexes.

Natalia A Kolganova1, Anna M Varizhuk, Roman A Novikov

  • 1a Engelhardt Institute of Molecular Biology; Russian Academy of Sciences; Moscow, Russia.

Artificial DNA, PNA & XNA
|December 9, 2014
PubMed
Summary
This summary is machine-generated.

Modified thrombin-binding aptamers (TBAs) show improved stability. Anomeric modifications in TGT loops enhance nuclease resistance while retaining anticoagulant properties, offering potential therapeutic benefits.

Keywords:
anomeranticoagulant propertiesnuclease resistancequadruplexthombin binding aptamer

More Related Videos

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

10.2K

Related Experiment Videos

Last Updated: Apr 19, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

10.2K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medicinal Chemistry

Background:

  • Thrombin-binding aptamer (TBA) is a DNA oligomer that inhibits thrombin by forming a G-quadruplex structure.
  • Native TBA is susceptible to rapid degradation by nucleases in vivo, limiting its therapeutic applications.
  • Previous studies have explored modifications to enhance TBA's nuclease resistance.

Purpose of the Study:

  • To investigate the effects of anomeric modifications on TBA structure, stability, and anticoagulant activity.
  • To assess the potential of modified TBAs as nuclease-resistant anticoagulant agents.

Main Methods:

  • Synthesis of chimeric aptamers incorporating non-natural α-anomers at specific nucleotide positions.
  • Structural analysis to confirm G-quadruplex formation and stability.
  • Assessment of anticoagulant activity using relevant assays.
  • Evaluation of nuclease resistance in vitro.

Main Results:

  • Anomeric modification of TT loops (at T4 and T13) significantly stabilized the G-quadruplex structure.
  • Replacement of core guanines disrupted quadruplex assembly or induced G-tetrad rearrangement.
  • Modification of the TGT loop enhanced nuclease resistance substantially without compromising anticoagulant activity.
  • Intact TT loops were crucial for retaining anticoagulant properties in chimeric aptamers.

Conclusions:

  • Anomeric modifications represent a viable strategy for enhancing TBA nuclease resistance.
  • TGT loop modification offers a promising approach to develop stable and effective anticoagulant aptamers.
  • Further development of anomerically modified TBAs could lead to improved therapeutic agents.