Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

1.4K
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
1.4K
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

4.6K
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
4.6K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

1.0K
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
1.0K
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

4.5K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
4.5K
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

2.3K
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
2.3K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

1.2K
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Negative design enables cell-free expression and folding of designed transmembrane β-barrels.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Stereoselectivity and functional plasticity of a common ligand-binding pocket in TRPM3.

Nature communications·2026
Same author

Small angle X-ray scattering reveals polyethylene glycol corona architecture in liposomes: influence of polyethylene glycol chain length, surface density and coating strategy.

Journal of colloid and interface science·2026
Same author

Studies of the structural properties and stability of the molybdenum transport protein ModA from Oleidesulfovibrio alaskensis G20 upon metal binding.

Archives of biochemistry and biophysics·2025
Same author

Multi-Stimulus Soft Actuators from Aerosol Jet Printed MXene-Cellulose Composite.

Nano letters·2025
Same author

Compact polyethylenimine-complexed mRNA vaccines.

Nature nanotechnology·2025
Same journal

Quantitative analysis of light-induced ion segregation in mixed-halide perovskites.

Journal of applied crystallography·2026
Same journal

Towards machine-learning-based on-the-fly analysis of neutron reflectometry.

Journal of applied crystallography·2026
Same journal

<i>mcstas_gisans</i>: combining ray tracing with the distorted-wave Born approximation using <i>McStas</i> and <i>BornAgain</i> for virtual GISANS experiments.

Journal of applied crystallography·2026
Same journal

Computational methods for automated center determination in electron diffraction patterns.

Journal of applied crystallography·2026
Same journal

Epitaxy of ultrathin Fe<sub>3</sub>O<sub>4</sub> films on SrTiO<sub>3</sub>(001): influence of growth parameters on the formation of coexisting (111)- and (001)-oriented phases.

Journal of applied crystallography·2026
Same journal

Spin excitations near the pressure-induced antiferromagnetic transition in SrCu<sub>2</sub>(BO<sub>3</sub>)<sub>2</sub>.

Journal of applied crystallography·2026
See all related articles

Related Experiment Video

Updated: Apr 19, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.6K

New developments in the ATSAS program package for small-angle scattering data analysis.

Maxim V Petoukhov1, Daniel Franke1, Alexander V Shkumatov1

  • 1European Molecular Biology Laboratory, Hamburg Unit, EMBL c/o DESY, Notkestrasse 85, Hamburg 22607, Germany.

Journal of Applied Crystallography
|December 9, 2014
PubMed
Summary
This summary is machine-generated.

The ATSAS software (version 2.4) now offers advanced tools for small-angle scattering data analysis. This update enhances 3D modeling, data processing, and accessibility for structural biology research.

Keywords:
ATSASbiological macromoleculescomputer programsdata analysisisotropic scatteringsmall-angle scatteringstructural modelling

More Related Videos

Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

10.2K
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

13.2K

Related Experiment Videos

Last Updated: Apr 19, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.6K
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

10.2K
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

13.2K

Area of Science:

  • Structural Biology
  • Biophysical Chemistry
  • Computational Biology

Background:

  • Small-angle X-ray scattering (SAXS) and neutron scattering (SANS) are powerful techniques for studying macromolecular structure in solution.
  • The ATSAS program package is a widely used tool for processing and analyzing SAXS/SANS data.
  • Continuous development is essential to incorporate new algorithms and improve user experience.

Purpose of the Study:

  • To introduce the new features and improvements in ATSAS version 2.4.
  • To enhance the capabilities for analyzing complex biological systems using SAXS/SANS data.
  • To improve the accessibility and usability of the ATSAS software package.

Main Methods:

  • Development of multiplatform data manipulation and display tools.
  • Implementation of automated data processing and parameter calculation programs.
  • Integration of high- and low-resolution models from complementary structural methods.
  • Introduction of novel algorithms for ab initio 3D model generation of oligomeric systems and complexes.
  • Enhancement of tools for analyzing data from biological mixtures and flexible systems.

Main Results:

  • ATSAS version 2.4 provides enhanced capabilities for processing and analyzing isotropic small-angle X-ray and neutron scattering data.
  • New algorithms facilitate the construction of 3D models for weakly interacting oligomeric systems and complexes.
  • Improved tools are available for analyzing complex biological mixtures and flexible macromolecules.
  • The release includes installers for major platforms (Windows, Linux, Mac OSX) and updated documentation.
  • Web-based developments include a user forum and expanded online access to ATSAS programs.

Conclusions:

  • ATSAS version 2.4 represents a significant advancement in the analysis of SAXS/SANS data.
  • The new features empower researchers to tackle more complex structural biology problems.
  • Enhanced accessibility and user support foster wider adoption and collaboration in the field.