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Purification and some properties of a 2Fe ferredoxin in Pseudomonas ovalis
D Ohmori1, F Yamakura, K Suzuki
1Department of Chemistry, School of Medicine, Juntendo University, Chiba, Japan.
Biochemistry International
|March 1, 1989
Abstract:
A [2Fe-2S] ferredoxin was found in Pseudomonas ovalis which was grown in a medium supplemented with glucose and ammonium sulfate. The molecular weight of the 2Fe ferredoxin was estimated to be 13,000. It contained 2.2 gramatoms of non-heme iron and 2.3 gramatoms of acid-labile sulfur per mole protein. The absorption and circular dichroism spectra were characteristic of those of [2Fe-2S] type ferredoxins, especially adrenodoxin and putidaredoxin. The electron paramagnetic resonance spectrum of the reduced protein showed an axial symmetry (g = 2.020, g = 1.939). The amino acid composition was determined.