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Method validation of the biochip array technology for synthetic cannabinoids detection in urine
Marisol S Castaneto1, Nathalie A Desrosiers, Kayla Ellefsen
1Chemistry & Drug Metabolism, National Institute on Drug Abuse, NIH, Baltimore, MD. USA.
Background:
Synthetic cannabinoids (SC) are widely-abused cannabimimetic drugs that do not screen positive in traditional cannabinoids immunoassays, making detection difficult.
Methods And Results:
The first commercially-available immunoassay for urinary SC was validated. Limits of detection (5-20 µg/L), imprecision (<13.1% intra-, <37.7% inter-assay), and cross-reactivity profiles of 22 SC and 37 metabolites were obtained. A large negative bias (-80.8 to -28.0%) was observed. Sensitivity (98.3%), specificity (48.1%) and efficiency (53.9%) were determined from screening 20,017 urine specimens and confirming 1432 presumptive positive and 1069 selected negative specimens by LC-MS/MS. Cutoff optimization improved performance to 87.6% sensitivity, 85.2% specificity, and 85.4% efficiency.
Conclusion:
This high-throughput urine SC assay has good sensitivity and improved specificity and efficiency at modified cutoff concentrations.
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