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Quantitative analysis of bisphosphonates in biological samples.
Veniamin N Lapko1, Patrick S Miller, Curtis E Sheldon
1Celerion, 621 Rose Street, Lincoln, NE USA 68502.
Bioanalysis
|December 9, 2014
Summary
On-column derivatization using diazomethane revolutionized bisphosphonate bioanalysis. This review covers methods for analyzing bisphosphonate drugs in biological samples using this technique.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Biochemistry
Background:
- Bisphosphonate drug bioanalysis presents significant challenges due to complex factors.
- Traditional methods struggle with sample clean-up and detection sensitivity for bisphosphonates.
Purpose of the Study:
- To review published methods for bisphosphonate bioanalysis using on-column derivatization.
- To discuss critical factors for the successful application of this technique in biological matrices.
Main Methods:
- Focuses on 'on-column' derivatization techniques for bisphosphonate analysis.
- Highlights the use of diazomethane and trimethylsilyl diazomethane as methylation reagents.
- Emphasizes liquid-chromatography-tandem mass spectrometry (LC-MS/MS) for detection.
Main Results:
- On-column derivatization transforms bisphosphonates into detectable species for LC-MS/MS.
- The methodology significantly improves biological sample clean-up.
- Successfully applied to the bioanalysis of numerous bisphosphonate drugs since 2006.
Conclusions:
- On-column derivatization is a pivotal technique for bisphosphonate bioanalysis.
- The method offers enhanced sensitivity and sample preparation efficiency.
- Continued application and refinement of this approach are crucial for therapeutic drug monitoring.
