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Updated: Apr 19, 2026

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
A rapid, simple, and inexpensive method for the preparation of strand-specific RNA-Seq libraries
1Department of Plant and Soil Sciences, University of Kentucky, 301A Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546-0312, USA, aghunt00@uky.edu.
Abstract:
High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.

