Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbial amylolytic enzymes.

M Vihinen1, P Mäntsälä

  • 1Department of Biochemistry, University of Turku, Finland.

Critical Reviews in Biochemistry and Molecular Biology
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Substitution scanning identifies a novel, catalytically active ibrutinib-resistant BTK cysteine 481 to threonine (C481T) variant.

Leukemia·2016
Same author

Somatic mutation databases as tools for molecular epidemiology and molecular pathology of cancer: proposed guidelines for improving data collection, distribution, and integration.

Human mutation·2008
Same author

Recommendations for locus-specific databases and their curation.

Human mutation·2007
Same author

A structured simple form for ordering genetic tests is needed to ensure coupling of clinical detail (phenotype) with DNA variants (genotype) to ensure utility in publication and databases.

Human mutation·2007
Same author

The structural basis of hyper IgM deficiency - CD40L mutations.

Protein engineering, design & selection : PEDS·2007
Same author

cDNA microarray analysis of gene expression in coeliac disease jejunal biopsy samples.

Journal of autoimmunity·2004
Same journal

Conformational mobility of cationic voltage-gated ion channels.

Critical reviews in biochemistry and molecular biology·2026
Same journal

DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.

Critical reviews in biochemistry and molecular biology·2026
Same journal

The impact of HIV-1 Nef on CD4<sup>+</sup> T cells.

Critical reviews in biochemistry and molecular biology·2026
Same journal

Recent insights into bacterial ESCRT-III-mediated membrane remodeling.

Critical reviews in biochemistry and molecular biology·2026
Same journal

CRISPR-Cas9 editing of agricultural crops and medicinal plants: toward a cornucopia of natural products.

Critical reviews in biochemistry and molecular biology·2025
Same journal

RNA modifications as a means of self-recognition and immune protection.

Critical reviews in biochemistry and molecular biology·2025
See all related articles

Microbial amylolytic enzymes break down starch into glucose. Their genetics, properties, and gene cloning are detailed, with alpha-amylase in Bacillus subtilis being well-understood.

Area of Science:

  • Biochemistry
  • Microbiology
  • Enzymology

Background:

  • Starch hydrolysis requires multiple microbial amylolytic enzymes.
  • These enzymes include alpha-amylase, beta-amylase, glucoamylase, alpha-glucosidase, pullulan-degrading enzymes, exoacting enzymes, and cyclodextrin glycosyltransferase.
  • Enzyme properties are influenced by the producing organism's environment.

Purpose of the Study:

  • To describe the features, action patterns, and physicochemical properties of various starch-degrading enzymes.
  • To review the occurrence, genetics, and gene cloning results for amylolytic enzymes.
  • To highlight the well-characterized alpha-amylase genetics in Bacillus subtilis.

Main Methods:

  • Review of literature on amylolytic enzymes.
  • Analysis of enzyme properties, action patterns, and occurrence.

Related Experiment Videos

  • Examination of genetic regulation and gene cloning data.

    • Main Results:

    • Genes for all discussed amylolytic enzyme groups have been cloned, revealing conserved regions crucial for function.
    • The genetics of alpha-amylase in Bacillus subtilis are extensively studied, involving synergistic genetic elements.
    • Glucoamylase exhibits multiple forms, likely due to glycosylation, proteolysis, mRNA splicing, and multiple genes.

    Conclusions:

    • Amylolytic enzymes are diverse, with varied properties and genetic regulation.
    • Gene cloning has identified conserved regions essential for enzyme structure and function.
    • Further research into glucoamylase heterogeneity and genetic regulation is warranted.