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Related Experiment Videos

Complete foldback transposable elements encode a novel protein found in Drosophila melanogaster.

N S Templeton1, S S Potter

  • 1Children's Hospital Research Foundation, Division of Basic Science Research, Cincinnati, OH 45229.

The EMBO Journal
|June 1, 1989
PubMed
Summary

Researchers analyzed a complete foldback (FB) transposable element, identifying novel DNA sequences and proteins. This study sheds light on the regulation of FB transposition in Drosophila melanogaster.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Transposable elements (TEs) are mobile DNA sequences that can alter an organism's genome.
  • Foldback (FB) transposable elements are a class of TEs found in Drosophila melanogaster.
  • Complete FB elements may encode proteins essential for their own regulation and transposition.

Purpose of the Study:

  • To analyze a complete foldback (FB) transposable element homologous to FB white-crimson (FBwc).
  • To identify and characterize potential regulatory proteins encoded by the FB element.
  • To investigate the genomic location and expression of these proteins.

Main Methods:

  • DNA sequencing of the central loop region and junctions of the FB element.
  • Bioinformatic screening of novel DNA sequences against the Gene data bank.

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  • Antibody production against a peptide from the longest open reading frame (ORF1).
  • Affinity chromatography to isolate the protein encoded by ORF1.
  • Immunohistochemical analysis to determine protein localization in Drosophila.

    • Main Results:

    • A complete FB element was analyzed, revealing a central DNA loop and novel inverted repeats at the junctions.
    • Three open reading frames (ORFs) were identified within the loop region.
    • No significant homology was found for the loop or junction sequences in existing databases.
    • A 71,000-dalton protein, corresponding to ORF1, was successfully isolated from early-stage embryo extracts.
    • Immunohistochemistry localized this protein to egg chambers in adult flies.

    Conclusions:

    • The analyzed FB element contains unique DNA sequences with potential regulatory functions.
    • The identified 71 kDa protein is likely involved in the regulation of FB transposition.
    • The protein's localization in egg chambers suggests a role in early development or germline transmission of the element.