Related Experiment Video
Updated: Apr 19, 2026

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy
Published on: March 28, 2025
Gaussian beam in two-photon fluorescence imaging of rat brain microvessel
Lingyan Shi1, Adrián Rodríguez-Contreras2, Robert R Alfano3
1The City College of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York, New York 10031, United StatesbThe City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United StatescThe City Colle.
Abstract:
The critical optical properties of a Gaussian laser beam in two-photon or multiphoton fluorescence imaging, including the beam spot size, depth of focus, and intensity profile, are investigated for spatially locating nanoscale solutes in and surrounding the microvessels of rat brain.

