Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

What's past is prologue: epigenetic memory links transient inflammation to future disease.

Signal transduction and targeted therapy·2026
Same author

Urea-cycle control of fibroblast energetics after myocardial infarction.

Cardiovascular research·2026
Same author

Rezatapopt and the Return of Targetable p53.

MedComm·2026
Same author

Mechanism of Mutation in G Protein-Gated Inwardly Rectifying K<sup>+</sup> Channel in Familial Hyperaldosteronism-Type III: Residue Fluctuations and Conformational Instability.

Molecules (Basel, Switzerland)·2026
Same author

Human DNA methylation biomarkers in diabetic complications: from association to clinical translation.

Epigenomics·2026
Same author

Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking dataset.

iScience·2026
Same journal

Sinomenine Regulates the TRIM32/IRF1/TRAF6 Axis to Inhibit Pyroptosis in Atopic Dermatitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Urolithin A Mitigates Renal Fibrosis by Promoting Fatty Acid Oxidation Through Orchestrating β-Catenin Signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

MiR-124 Inhibits Lipid Deposition in Mouse Liver by Targeting the Trib3/Hnf4α Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

TCN2 Drives Psoriasis-Like Inflammation and Keratinocyte Hyperproliferation, Correlating With IL-1β and STAT3 Activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

The Synthetic Melanocortin Agonist NDP-MSH Ameliorates THSD7A-Associated Membranous Nephropathy in an Active Immunization Mouse Model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Brain-Derived Neurotrophic Factor and Associated Signaling in Kidney Diseases.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
See all related articles

Related Experiment Video

Updated: Apr 19, 2026

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.8K

Dynamic changes in the cardiac methylome during postnatal development.

Choon Boon Sim1, Mark Ziemann1, Antony Kaspi1

  • 1*School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia; and Epigenetics in Human Health and Disease Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|December 11, 2014
PubMed
Summary
This summary is machine-generated.

DNA methylation guides postnatal heart maturation by regulating gene expression. Inhibiting DNA methylation in neonatal mice increased cardiomyocyte proliferation, suggesting epigenetic control over cell cycle arrest.

Keywords:
DNA methylationbinucleationcardiomyocyte proliferationepigeneticsneonatal heart

More Related Videos

Detection of Global DNA Methylation in the Hearts of Zebrafish Larvae
05:07

Detection of Global DNA Methylation in the Hearts of Zebrafish Larvae

Published on: April 10, 2026

16
Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

4.2K

Related Experiment Videos

Last Updated: Apr 19, 2026

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.8K
Detection of Global DNA Methylation in the Hearts of Zebrafish Larvae
05:07

Detection of Global DNA Methylation in the Hearts of Zebrafish Larvae

Published on: April 10, 2026

16
Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

4.2K

Area of Science:

  • Epigenetics
  • Developmental Biology
  • Cardiovascular Science

Background:

  • Postnatal organ maturation involves complex epigenetic regulation.
  • Understanding epigenetic control in heart development is crucial for cardiomyocyte maturation and loss of regenerative potential.

Purpose of the Study:

  • To investigate the role of DNA methylation in transcriptional changes during the first two weeks of mouse heart development.
  • To determine if DNA methylation regulates cardiomyocyte maturation, proliferation, and cell cycle arrest.

Main Methods:

  • Gene expression profiling using RNA-sequencing (RNA-seq).
  • Genome-wide sequencing of methylated DNA (MBD-seq) to identify differentially methylated regions (DMRs).
  • Pharmacological inhibition of DNA methylation using 5-aza-2'-deoxycytidine.

Main Results:

  • Identified 2545 differentially methylated regions (DMRs) in the cardiac methylome from postnatal day 1 to 14.
  • Observed widespread hypermethylation (~80% of DMRs) associated with the shutdown of key developmental signaling pathways (Hedgehog, BMP, TGF-β, FGF, Wnt/β-catenin).
  • Inhibition of DNA methylation led to a 3-fold increase in cardiomyocyte proliferation and a 50% reduction in binucleated cardiomyocytes.

Conclusions:

  • DNA methylation undergoes dynamic changes during postnatal mouse heart development.
  • Epigenetic regulation via DNA methylation is critical for suppressing cardiomyocyte proliferation and promoting maturation.
  • Targeting DNA methylation may offer therapeutic strategies for enhancing cardiac regeneration.