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A novel effect of parylene-based surface coating on HepG2 cell function.

Hideto Tozawa¹, Toshiro Maekawa², Hiroshi Kimura¹

¹Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.

Materials Science & Engineering. C, Materials for Biological Applications

|December 11, 2014

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Parylene-C AM (diX AM) coating promotes HepG2 cell adhesion and induces CYP1A1/CYP1A2 gene expression, demonstrating a novel effect on cell function. This biocompatible material offers a non-animal alternative for cell culture applications.

Area of Science:

Biomaterials Science
Cell Biology
Hepatocellular Carcinoma Research

Background:

Parylene-C (diX C) is a biocompatible surface coating with diverse biological applications.
Parylene-C AM (diX AM), a variant of diX C, exhibits high cell adhesiveness, but its impact on cell function is unknown.

Purpose of the Study:

To investigate the effect of diX AM on the morphology and gene expression of human hepatocellular carcinoma (HepG2) cells.
To explore the potential of diX AM as a non-animal-derived material for cell culture.

Main Methods:

Culturing HepG2 cells on diX AM surfaces.
Assessing cell morphology.
Performing microarray analysis to examine gene expression.
Measuring CYP1 enzymatic activity using the ethoxyresorufin-O-dealkylase (EROD) assay.

Keywords:

Cell adhesion Collagen Cytochrome P450 Gene expression Parylene Surface treatment

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Main Results:

HepG2 cells adhered to diX AM surfaces, maintaining morphology comparable to cells on collagen-coated surfaces.
Microarray analysis revealed significant induction of CYP1A1 and CYP1A2 gene expression in HepG2 cells cultured on diX AM.
CYP1 enzymatic activity, assessed via EROD assay, correlated with the observed gene expression induction.

Conclusions:

diX AM exerts a novel effect on HepG2 cell function, specifically inducing CYP1A1 and CYP1A2 expression and activity.
diX AM demonstrates potential as a non-animal-derived material suitable for cell culture applications.
These findings open new avenues for using diX AM in biomedical research and tissue engineering.