Total synthesis of elastin peptide using high pressure-liquid phase synthesis assisted by a soluble tag strategy

A highly aggregating elastin peptide was prepared efficiently using a high pressure-liquid phase synthesis approach assisted by a soluble tag strategy. Two standard syringes were connected to each other to construct a reactor. This simple reactor was used to apply high pressure to the highly viscous reaction mixture thereby maintaining its fluidity. The reactions were completely inhibited due to aggregation when conducted in a standard flask reactor, whereas our high pressure approach accelerated the couplings to realize complete conversion within 5-7 min. All steps were conducted at 0.10 M concentration, affording grams of the desired product.