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Related Experiment Video

Updated: Apr 19, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

Super-resolution imaging in live cells.

¹Randall Division of Cell and Molecular Biophysics, King׳s College London, SE1 1UL, UK.

Developmental Biology

|December 16, 2014

Summary

This summary is machine-generated.

Super-resolution microscopy offers tenfold resolution improvements for cellular imaging. This review details live-cell adaptations of stimulated emission depletion, structured illumination, and localization microscopy, addressing speed and phototoxicity challenges.

Keywords:

Localisation microscopy Stimulated emission depletion microscopy Structured illumination microscopy Super-resolution microscopy

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Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Super-resolution fluorescence microscopy has advanced significantly over 20 years.
Commercial systems are now widely available, offering >10x resolution improvement.
Key techniques include stimulated emission depletion (STED), structured illumination microscopy (SIM), and localization microscopy (LM).

Purpose of the Study:

To review super-resolution microscopy techniques suitable for live-cell imaging.
To discuss the trade-offs between resolution, speed, and implementation for each method.
To evaluate the quality of results achievable in live cells.

Main Methods:

Discussion of STED, SIM, and LM for live-cell applications.
Analysis of challenges including phototoxicity and imaging speed.
Comparison of performance characteristics for different super-resolution approaches.

Main Results:

Live-cell super-resolution imaging is achievable with STED, SIM, and LM.
Significant challenges remain in optimizing speed and minimizing phototoxicity.
Trade-offs exist between resolution, acquisition speed, and ease of use for each technique.

Conclusions:

Live-cell super-resolution microscopy requires careful consideration of technique-specific limitations.
Ongoing development aims to improve speed and reduce phototoxicity for routine live-cell applications.
The choice of technique depends on the specific research question and experimental constraints.