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Detection of plasmid-mediated quinolone resistance determinants and qnrS expression in Enterobacteriaceae clinical
Xiaobing Jiang1, Jun Li, Yunshan Zhang
1College of Life Sciences, Henan Normal University, Xinxiang, China. jxb841001@163.com.
Introduction:
Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates.
Methodology:
A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genes using polymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS.
Results:
The rates of qnr and aac(6')-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present.
Conclusions:
Our data suggest that the level of qnrS expression was associated with quinolone resistance.
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