Related Experiment Video
Updated: Sep 12, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Development of a rapid isothermal amplification method for clinical detection of Vibrio cholerae
Yan Shen1, Si-Ming Lu1, Hong-Liang Liu2
1Department of Laboratory Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Introduction:
The aim of this study was to develop a rapid isothermal amplification assay for Vibrio cholerae.
Methodology:
Specific primers were designed following the guidelines of the National Center for Biotechnology Information (NCBI) database and based on the highly conserved sequence of the Vibrio cholerae virulence factor gene ctxA. A detection method was then developed using recombinase polymerase amplification (RPA). Sensitivity, specificity, clinical sample collection, and processing were calculated.
Results:
A rapid isothermal amplification system was developed, showing a detection capability of one copy per microliter. Specificity analysis against a panel of control strains yielded no false-positive results under the tested conditions. The presence of V. cholerae was detected in 9 fecal samples collected from 20 patients who were hospitalized with suspected cholera infection.
Conclusions:
This method can be an effective aid in the detection of bacteria in clinical samples.
More Related Videos
Related Concept Videos
Automated Microbial Diagnostics
Cholera
Rapid Identification of Pathogens

