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Epstein-Barr virus replication in interferon-treated cells.

N A Sharp1, J R Arrand, M J Clemens

  • 1Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London, U.K.

The Journal of General Virology
|September 1, 1989
PubMed
Summary
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Human interferon-alpha (IFN) inhibits growth and induces differentiation in Epstein-Barr virus (EBV)-infected cells, but does not significantly activate EBV replication. IFN partially inhibits chemically induced EBV replication in these cells.

Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Daudi cells, derived from Burkitt's lymphoma, are latently infected with Epstein-Barr virus (EBV).
  • Human interferon-alpha (IFN) exhibits potent growth inhibitory and differentiation-inducing effects on Daudi cells.

Purpose of the Study:

  • To investigate whether the effects of IFN on Daudi cells are mediated by the induction of EBV replication.
  • To determine the impact of IFN on EBV lytic gene expression and viral DNA replication.

Main Methods:

  • Treatment of EBV-infected Daudi cells with human interferon-alpha (IFN).
  • Analysis of EBV lytic gene expression at RNA and protein levels.
  • Chemical induction of EBV replication.
  • Quantification of viral DNA copy number.

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Main Results:

  • IFN treatment induced complete cell growth inhibition and differentiation in <0.3% of cells expressing EBV lytic genes.
  • Concomitant IFN treatment partially inhibited the increase in EBV lytic gene transcripts and viral DNA copy number during chemical induction.
  • IFN had no effect on viral DNA levels in uninduced cells.

Conclusions:

  • The growth inhibitory and differentiation effects of IFN on Daudi cells are largely independent of EBV lytic replication induction.
  • IFN partially suppresses chemically induced EBV replication, suggesting an antiviral role beyond direct lytic gene activation.