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Temporal Bacteriostatic Effect and Growth Factor Loss in Equine Platelet Components and Plasma Cultured with

Catalina López1, María E Alvarez1, Jorge U Carmona1

  • 1Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.

Veterinary Medicine International
|December 16, 2014
PubMed

Insights

Platelet-rich plasma (PRP) and its derivatives show significant bacteriostatic effects against Staphylococcus aureus, including MRSA. However, methicillin-resistant strains exhibited higher growth in some components over 24 hours.

Area of Science:

  • Veterinary Medicine
  • Microbiology
  • Biochemistry

Background:

  • Platelet-rich plasma (PRP) and its derivatives are utilized in equine medicine for their regenerative properties.
  • The antimicrobial efficacy of these blood components against bacterial pathogens like Staphylococcus aureus requires further investigation.
  • Understanding the interaction between PRP components and bacterial growth is crucial for optimizing therapeutic applications.

Purpose of the Study:

  • To evaluate the bacteriostatic effects of various equine blood components, including PRP, on methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus.
  • To determine the concentrations and degradation of key growth factors (PF-4, TGF-β1, PDGF-BB) within these components over 24 hours.
  • To explore correlations between bacterial growth and the concentrations of cellular factors and growth factors in equine blood components.

Main Methods:

  • Equine blood components (PRP, PRG, LPP, LPG, plasma, IP) were prepared using the tube method.
  • Components were co-cultured with MSSA and MRSA, and bacterial growth was assessed at 6 and 24 hours.
  • Concentrations of platelet factor 4 (PF-4), TGF-β1, and PDGF-BB were measured at the same time points.

Main Results:

  • All tested blood components, except heat-inactivated plasma (IP), significantly inhibited bacterial growth at 6 hours, with MSSA being more sensitive than MRSA.
  • At 24 hours, bacterial growth was significantly higher in IP, and MRSA showed higher growth than MSSA in PRP, LPP, and plasma.
  • Bacterial presence did not significantly alter the concentrations of PF-4, TGF-β1, or PDGF-BB in the tested blood components.

Conclusions:

  • Equine PRP and its derivatives possess bacteriostatic properties against Staphylococcus aureus, though efficacy may vary with bacterial strain and incubation time.
  • The study highlights potential differences in the susceptibility of MSSA and MRSA to equine blood components.
  • Growth factors within these components do not appear to be significantly degraded by bacterial interaction within the study period.

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