[Human lymphocyte immunophenotype after interaction with mesenchymal stromal cells]

Human multipotent mesenchymalstromal cells were cocultured for 72h with allogeneic blood-borne mononuclear cells (MNCs) of different maturity (lymphocytes from adult peripheral blood and umbilical cord blood) and functional state (intact and PHA-activated human peripheral blood lymphocytes): After coculture with MMSCs the share ofB-cells among MNCs and cbMNCs decreased. The proportion ofT- and NK cells declined only among cbMNCs (p < 0.05). Only T-NK subpopulation reduced among MNC and cbMNC T-cells. The drop of CD8+ cells was detected in coculture of MMSCs and PHA-MNCs. Activated HLA-DR+ cells declined, CD25+ cells increased compared to monoculture of PHA-MNCs. MMSCs supported MNC, PHA-MNC and cbMNC viability. These results are important for understanding the physiology of the allogeneic cell-to-cell interaction in connection of potential clinical application of allogeneic cell products of blood-borne and stroinal origin.