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Updated: Aug 3, 2026
Digital Microfluidics for Automated Proteomic Processing
Published on: November 6, 2009
Gel-aided sample preparation (GASP)--a simplified method for gel-assisted proteomic sample generation from protein
Roman Fischer1, Benedikt M Kessler
1TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Abstract:
We describe a "gel-assisted" proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types, and accessible to nonspecialists.
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