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Plaque Assay for Murine Norovirus
Published on: August 22, 2012
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A simple and highly repeatable viral plaque assay for enterovirus 71
Yingxian Yin1, Yi Xu, Zhiying Ou
1Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou, China.
Journal of Basic Microbiology
|December 18, 2014
Summary
This study introduces a simplified Avicel-based plaque assay for counting infectious viral particles. This improved method enhances repeatability and ease of use in virological experiments, making viral quantification more accessible.
Area of Science:
- Virology
- Cell Biology
- Biotechnology
Background:
- The traditional plaque assay is crucial for quantifying infectious viral particles but is often complex and difficult to repeat.
- Limitations in current methods hinder their application in diverse virological research settings.
Purpose of the Study:
- To develop a simplified and more repeatable plaque assay procedure.
- To optimize viral quantification using Enterovirus 71 (EV71) in Vero cells.
Main Methods:
- An improved plaque assay utilizing Avicel was developed.
- The assay was tested on Vero cells infected with Enterovirus 71 (EV71).
- Plaque visualization was achieved without the need for immunostaining.
Main Results:
- The improved Avicel-based plaque assay simplifies operations and enhances experimental repeatability.
- Visible plaques were formed on both 24-well and 96-well plates.
- The method demonstrated effectiveness with Enterovirus 71 in Vero cells.
Conclusions:
- The developed plaque assay offers a simpler and more repeatable alternative to traditional methods.
- This technique facilitates easier viral particle counting and can be adapted for various viruses.
- The assay's simplicity and repeatability make it suitable for a broader range of virological experiments.
