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DNA adsorption by indium tin oxide nanoparticles.

Biwu Liu1, Juewen Liu

  • 1Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.

Langmuir : the ACS Journal of Surfaces and Colloids
|December 19, 2014
PubMed
Summary

Indium tin oxide nanoparticles efficiently adsorb DNA via their phosphate backbone. This interaction, crucial for biosensor development, is tunable by surface charge and DNA properties, enabling DNA desorption through duplex formation.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Indium tin oxide (ITO) is widely used in electronics due to its conductivity and transparency.
  • Emerging applications of ITO in biosensors necessitate understanding its biointerface chemistry.
  • Investigating DNA adsorption on ITO nanoparticles (NPs) is key to exploring its biosensing potential.

Purpose of the Study:

  • To elucidate the biointerface chemistry between DNA and ITO NPs.
  • To understand the factors influencing DNA adsorption and desorption on ITO NPs.
  • To compare DNA interaction with ITO NPs to other metal oxide nanoparticles.

Main Methods:

  • Utilized fluorescently labeled single-stranded oligonucleotides.
  • Employed ITO nanoparticles (NPs) for interaction studies.

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  • Conducted displacement experiments using free phosphate and DNA bases.
  • Demonstrated DNA desorption via duplex DNA formation.

    • Main Results:

    • DNA fluorescence was quenched upon adsorption to ITO NPs.
    • DNA adsorption capacity is strongly dependent on ITO surface charge (pH) and DNA characteristics (sequence, length).
    • ITO's adsorption is an average of In2O3 (strong adsorption) and SnO2 (repulsion) components.
    • Adsorption primarily involves the DNA phosphate backbone.
    • DNA-induced DNA desorption via duplex formation was demonstrated on ITO, unlike other metal oxides.

    Conclusions:

    • ITO NPs exhibit significant DNA adsorption, primarily through the phosphate backbone, influenced by surface charge and DNA properties.
    • The unique DNA adsorption-desorption behavior on ITO, particularly DNA-induced desorption, offers advantages for biosensing applications.
    • ITO NPs present a promising platform for biosensors, with tunable DNA interactions compared to other metal oxides.