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Expression and Function of LPA1 in Bladder Cancer
Masao Kataoka1, Kei Ishibashi1, Shin Kumagai1
1Department of Urology, Fukushima Medical University, Fukushima, Japan; Department of Pathology (HC), Fukushima Medical University, Fukushima, Japan.
The Journal of Urology
|December 20, 2014
Summary
Lysophosphatidic acid (LPA) signaling through LPA receptor 1 (LPA1) promotes bladder cancer invasion. LPA1 may serve as a biomarker for detecting invasive bladder cancer and a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Lysophosphatidic acid (LPA) is a lipid mediator promoting cell proliferation and invasion.
- LPA receptors, a class of G-protein coupled receptors, are potential therapeutic targets in malignant cancers.
Purpose of the Study:
- To evaluate LPA receptor expression in bladder cancer.
- To investigate the effect of LPA on bladder cancer cell invasion.
Main Methods:
- Real-time PCR and immunohistochemical staining to determine LPA receptor expression in bladder cancer tissues.
- In vitro assays using T24 bladder cancer cells to assess LPA effects on invasion, ROCK expression, and myosin light chain phosphorylation.
Main Results:
- LPA receptor 1 (LPA1) mRNA expression was significantly higher in muscle-invasive bladder cancer.
- LPA treatment increased T24 cell invasion, ROCK1 expression, and myosin light chain phosphorylation.
- LPA1 knockdown or inhibition reduced LPA-induced cell invasion.
Conclusions:
- LPA signaling via LPA1 activation promotes bladder cancer invasion.
- LPA1 may be a useful biomarker for detecting highly invasive bladder cancer.
- LPA1 represents a potential therapeutic target for invasive bladder cancer treatment.
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