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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Versatile microfluidic droplets array for bioanalysis.

Shan-Wen Hu¹, Bi-Yi Xu, Wei-Ke Ye

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, P.R. China.

ACS Applied Materials & Interfaces

|December 20, 2014

Summary

This summary is machine-generated.

We developed a novel hydrophilic chip for versatile droplet arrays, enabling rapid liquid handling and gradient concentration generation. This method enhances convenience and versatility for high-throughput bioscreening and biosensing applications.

Keywords:

droplets array liquid dispensation rapid analysis surface wettability

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Area of Science:

Microfluidics
Biotechnology
Materials Science

Background:

Droplet arrays are crucial for high-throughput biological assays.
Current methods for generating droplet arrays can be complex and time-consuming.
Developing versatile and convenient platforms for droplet generation is essential for advancing bioscreening and biosensing.

Purpose of the Study:

To propose a novel method for creating versatile droplet arrays on a regional hydrophilic chip.
To demonstrate the chip's capability for rapid liquid dispensation and gradient concentration generation.
To showcase the application of this platform in high-throughput toxicological screening and cancer biomarker detection.

Main Methods:

Fabrication of a regional hydrophilic chip using PDMS soft lithography and regional plasma treatment.
Integration of a Christmas Tree structure for generating concentration gradients in droplet arrays.
Application in toxicological studies using Cadmium Selenide (CdSe) quantum dots on HL-60 cells.
Development of a magnetic beads (MBs)-based sandwich immunoassay for cancer biomarker detection.

Main Results:

Achieved versatile droplet array formation with rapid liquid dispensation.
Successfully generated droplet arrays with gradients in concentration.
Enabled quick and efficient counting of cell death rates in toxicological tests.
Demonstrated a rapid biosensing approach for detecting carcinoma embryonic antigen (CEA).

Conclusions:

The novel hydrophilic chip offers a convenient and versatile platform for droplet array generation.
The developed method significantly improves performance in bioscreening and biosensing.
This technology facilitates high-throughput toxicological screening and rapid detection of cancer biomarkers.