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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Nov 14, 2025

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Super-resolution plasmonic imaging via scattering saturation STED.

Zhaoshuai Gao¹, Pei Wu, Lixin Yin

¹State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Centre of Chemistry for Life Sciences, Nanjing University, 163 Xianlin Road, Nanjing 210023, China. binkang@nju.edu.cn xujj@nju.edu.cn.

Chemical Communications (Cambridge, England)

|March 10, 2021

Summary

This summary is machine-generated.

We developed a new super-resolution microscopy technique using nonlinear plasmonic scattering. This scattering saturation STED (ssSTED) microscope achieves a spatial resolution of 65 nm for detailed nanoscale imaging.

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Last Updated: Nov 14, 2025

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Area of Science:

Optics and Photonics
Nanotechnology
Microscopy

Background:

Super-resolution microscopy overcomes the diffraction limit for enhanced imaging.
Plasmonic scattering offers unique optical properties for nanoscale applications.
Stimulated emission depletion (STED) microscopy is a powerful super-resolution technique.

Purpose of the Study:

To develop a novel single-wavelength super-resolution imaging method.
To leverage nonlinear plasmonic scattering for improved spatial resolution.
To demonstrate the capability of the scattering saturation STED (ssSTED) microscope.

Main Methods:

Utilizing the nonlinear plasmonic scattering response of materials.
Employing modulated excitation in the time domain.
Implementing a custom-built scattering saturation STED (ssSTED) microscope system.

Main Results:

Achieved a spatial resolution of λ/7 (65 nm).
Successfully imaged 50 nm gold nanoparticles with high resolution.
Demonstrated the effectiveness of the ssSTED technique.

Conclusions:

The ssSTED microscope enables high-resolution imaging at a single wavelength.
Nonlinear plasmonic scattering is a viable mechanism for super-resolution microscopy.
This method advances nanoscale imaging capabilities.