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The Lingulodinium circadian system lacks rhythmic changes in transcript abundance.

Sougata Roy1, Mathieu Beauchemin2, Steve Dagenais-Bellefeuille3

  • 1Département de Sciences Biologiques, Université de Montréal, Institut de Recherche en Biologie Végétale, 4101 Sherbrooke est, Montréal, Québec, H1X 2B2, Canada. sougatairbv20@gmail.com.

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Circadian rhythms in unicellular dinoflagellates like Lingulodinium do not rely on daily fluctuations in RNA levels. This suggests their internal clock operates independently of rhythmic gene transcription, challenging traditional models of transcription-translation feedback loops.

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Area of Science:

  • * Molecular Biology
  • * Chronobiology
  • * Marine Biology

Background:

  • * Cells exhibit circadian rhythms, approximately 24-hour cycles in biological processes.
  • * These rhythms are controlled by an internal circadian clock, often involving transcription-translation feedback loops (TTFL).
  • * In TTFL, clock gene products inhibit their own transcription.

Purpose of the Study:

  • * To investigate the presence and mechanism of circadian rhythms in the unicellular dinoflagellate Lingulodinium.
  • * To determine if daily variations in transcript abundance correlate with circadian rhythms in this organism.
  • * To assess the role of transcription in maintaining circadian rhythms, specifically bioluminescence and photosynthesis.

Main Methods:

  • * Utilized RNA-Sequencing (RNA-Seq) to quantify transcript abundance in Lingulodinium.
  • * Analyzed transcript levels under both light-dark cycles and constant light conditions at various time points.
  • * Employed transcription inhibitors (actinomycin D, cordycepin) to study the impact on rhythm timing.

Main Results:

  • * No detectable daily variation in the abundance of any transcript was observed in the Lingulodinium gene catalog.
  • * Bioluminescence and photosynthesis rhythms persisted even when transcription rates were significantly inhibited.
  • * The timing of these rhythms remained unchanged despite a drastic reduction in transcription.

Conclusions:

  • * The circadian timer in Lingulodinium does not depend on rhythmic RNA levels.
  • * Cellular timekeeping in this organism may be regulated by mechanisms other than clock gene transcript fluctuations.
  • * Findings challenge the universal applicability of TTFL as the sole mechanism for circadian timekeeping.