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CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells

Richard Moore1, Alec Spinhirne2, Michael J Lai3

  • 1Bioengineering Department, University of Texas at Dallas, Richardson, TX 75080, USA Electrical Engineering Department, University of Texas at Dallas, Richardson, TX 75080, USA.

Nucleic Acids Research
|December 21, 2014
PubMed
Summary

This study introduces a novel CRISPR-Cas9 gene delivery system for precise control over gene product levels and vector clearance in human cells. This method enhances gene therapy and synthetic biology applications by enabling tunable gene expression and trace-free delivery.

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