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Profiling of serum and urinary microRNAs in children with atopic dermatitis
Yani Lv1, Ruiqun Qi1, Jing Xu2
1Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, China.
Background:
Atopic dermatitis (AD) is the most prevalent chronic inflammatory skin disease in children characterized by dermatitis and pruritus. MicroRNAs (miRNAs) have been shown as great potential biomarkers for disease fingerprints to predict prognostics. We aimed to identify miRNA signature from serum and urine for the prognosis of AD patient by genome-wide miRNA profiling analysis.
Methods:
Serum and urine from 30 children with AD and 28 healthy children were collected and their genome-wide miRNA expression profiles were measured by TaqMan-based array and confirmed by quantitative real-time PCR. Inflammatory factors in serum were detected by Antibody Array System.
Results:
miR-203 and miR-483-5p were significantly up-regulated in serum of children with AD compared with healthy children. The level of miR-483-5p in serum was significantly associated with other atopic conditions, such as rhinitis and/or asthma. However, miR-203 was markedly decreased in urine of children with AD compared with healthy children. Down-regulated miR-203 in urine was significant associated with abnormal level of serum IgE in AD patients. 7 inflammatory factors in serum were altered in children with AD compared with healthy children. Up-regulated miR-203 in serum was significantly associated with increased sTNFRI and sTNFRII.
Conclusions:
Up-regulated miR-483-5p in serum may be indicative of other atopic conditions in children with AD. Down-regulated miR-203 in urine may serve as a biomarker for the severity of inflammation in children with AD.
Insights
This study identified specific microRNAs (miRNAs) in children with atopic dermatitis (AD). Elevated serum miR-483-5p may indicate other allergic conditions, while decreased urinary miR-203 could predict inflammation severity in AD patients.
Area of Science:
- Biochemistry
- Genetics
- Immunology
Background:
- Atopic dermatitis (AD) is a common childhood inflammatory skin condition.
- MicroRNAs (miRNAs) show promise as biomarkers for predicting disease outcomes.
- Genome-wide miRNA profiling can identify diagnostic signatures.
Purpose of the Study:
- To identify miRNA signatures in serum and urine for atopic dermatitis (AD) prognosis in children.
- To analyze genome-wide miRNA expression profiles for AD biomarkers.
Main Methods:
- Collected serum and urine samples from 30 children with AD and 28 healthy controls.
- Performed genome-wide miRNA expression profiling using TaqMan-based arrays and confirmed with quantitative real-time PCR.
- Detected serum inflammatory factors using an Antibody Array System.
Main Results:
- miR-203 and miR-483-5p were significantly upregulated in the serum of children with AD.
- miR-483-5p levels correlated with other atopic conditions like rhinitis and asthma.
- miR-203 was downregulated in the urine of children with AD, correlating with serum IgE levels and increased sTNFRI/sTNFRII.
Conclusions:
- Upregulated serum miR-483-5p may indicate co-existing atopic conditions in children with AD.
- Downregulated urinary miR-203 could serve as a biomarker for inflammation severity in pediatric AD.
