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[Expressions of Bim and ERKs in multidrug-resistant hepatic carcinoma cells]

Feng Yan1, Xiaomin Wang, Quanming Ma

  • 1Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, Xiamen 361004, China.E-mail: yanfeng@xmzsh.com.

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|December 25, 2014
PubMed
Abstract

Insights

Extracellular signal-regulated kinase (ERK) and Bim expressions are linked to multidrug resistance in hepatocellular carcinoma (HCC) cells. Decreased Bim and altered ERK phosphorylation correlate with resistance, suggesting therapeutic targets for HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Hepatocellular carcinoma (HCC) often develops multidrug resistance (MDR), limiting treatment efficacy.
  • Understanding the molecular mechanisms underlying HCC drug resistance is crucial for developing novel therapeutic strategies.

Purpose of the Study:

  • To investigate the expression of extracellular signal-regulated kinase (ERK) and Bim in multidrug-resistant HCC cells.
  • To determine the association between ERK and Bim expression and drug resistance in HCC.

Main Methods:

  • Established a multidrug-resistant HepG-2 cell line (HepG-2/ADM) using Adriamycin (ADM).
  • Assessed drug sensitivity using CCK-8 assays.
  • Quantified expressions of MRP-1, P-gp, ERK1, ERK2, ERK5, and Bim via Western blot and quantitative real-time PCR.

Main Results:

  • HepG-2/ADM cells exhibited resistance to ADM, 5-FU, and CDDP.
  • Upregulation of MRP-1, P-gp, ERK1, ERK2, and ERK5 was observed.
  • Decreased Bim mRNA and protein levels, along with altered ERK phosphorylation patterns, were noted.

Conclusions:

  • ERK and Bim play a significant role in the development of multidrug resistance in HepG-2/ADM cells.
  • These findings suggest ERK and Bim as potential therapeutic targets for overcoming drug resistance in HCC.

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