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Updated: Apr 19, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Expression of gamma interferon during HPV and Chlamydia trachomatis infection in cervical samples]
María Del Carmen Colín-Ferreyra1, Hugo Mendieta-Zerón2, María Del Socorro Romero-Figueroa3
1Laboratorio de Biología Molecular, Centro de Investigación en Ciencias Médicas (CICMED), Posgrado en Ciencias de la Salud, Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, México.
Introduction:
The aim of this study was to mesure the expression of gamma interferon in HPV and Chlamydia trachomatis infection in squamous intraepithelial lesions.
Method:
Samples from 100 patients diagnosed by colposcopy with or without squamous intraepithelial lesions were used in the present study. Each patient was found to be infected by HPV and C.trachomatis. Relative gamma interferon mRNA expression was assessed using a real-time reverse transcriptase PCR assay (RT-PCR).
Results:
The relative units of expression of gamma interferon mRNA were 13, 1.8 and 0.3, for HPV and C.trachomatis co-infection, or HPV or C.trachomatis infection, respectively.
Conclusion:
HPV and C.trachomatis could overstimulate the expression of gamma interferon.
