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Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
Published on: May 24, 2024
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Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation
Jussimara Monteiro1, Fernanda Matsiko Inoue1, Ana Paula T Lobo1
1Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil.
Journal of Microbiological Methods
|December 27, 2014
Abstract:
A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively.
Keywords:
Bacterial identificationBlood cultureMALDI-TOFMass spectrometryTrifluoroacetic acidVitek MSMore Related Videos
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