[Alterations of immunebalance in the rats blood during experimental preeclampsia]

The aim of the study was to establish a violation of hormonal (progesterone and estradiol) and immune balance (interleukins IL-2, IL-10, TNF-α) in the rats blood at different stages of experimental PE induced by hypoxia placenta. For the purpose of modeling of PE in pregnant rats, at 10-thday of gestation the lumen of the abdominal aorta below the renal artery was narrowed by the silk thread a third of its diameter (0.2 mm). Blood for the study was taken at the18thand25th day of pregnancy. In the blood content of interleukins(IL-2, IL-10, TNF-α) and the concentration of progesterone and estradiol by immunoenzymaticassay were measured. A results of research established that with increasing of the gestational age in the rats with experimental PE estradiol and progesterone content in the blood increased much slower than in the control animals. At the same time in the blood of pregnant rats with placental hypoxia in the second trimester TNF-α level was by 80% higher and the level of IL-10 was 30% lower than in the control animals. With the increase of gestational age (in the third trimester) in animals of the experimental group blood content of IL-2 and TNF-α increased sharply (by 50% and 30%), whereas the level of IL-10 did not change compared with baseline values. In the control group of animals, alterations in the cytokines content were much slower. It was concluded that violation of the synthesis of progesterone in hypoxic placenta and its insufficient content in the blood during pregnancy is one of the causes of disorders of the immune balance in the body of pregnant rats. Observation of the dynamics of progesterone in the blood during pregnancy may be informative for the early diagnosis of PE and its timely treatment.