Related Experiment Video
Updated: Aug 9, 2026

In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats
Published on: September 27, 2017
Stimulation of Cl/HCO3 exchange in rat duodenal brush border membrane vesicles by cAMP
C R Dunk1, C D Brown, L A Turnberg
1Department of Medicine, Hope Hospital, University of Manchester School of Medicine, Salford, Great Britain.
Abstract:
Brush border membrane vesicles have been used to study the regulation of rat duodenal HCO3 secretion. When vesicles were loaded with cAMP and ATP SITS-sensitive Cl/HCO3 exchange (unidirectional 36Cl influx in response to an outward-facing OH/HCO3 gradient) was stimulated by approximately 25%. In contrast, there was no effect of cAMP upon SITS-insensitive 36Cl uptake. The stimulation of Cl/HCO3 exchange caused by cAMP was abolished in the presence of a specific heat-stable cAMP-dependent protein kinase inhibitor. These results suggest that Cl/HCO3 exchange, and hence HCO3 secretion, is regulated by cAMP via phosphorylation of some component of the membrane associated with the transport protein.
Related Concept Videos
GPCRs Regulate Adenylyl Cylase Activity
Two...
Intestinal Phase of Digestion
The arrival of the chyme in the small intestine distends the duodenum, which triggers the enterogastric reflex. This distension...

