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Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
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A group-specific sequence-based typing approach for HLA-DQA1
A J Lemin1, J Johnson, C Darke
1Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, UK.
International Journal of Immunogenetics
|December 30, 2014
Abstract:
An HLA-DQA1 sequence-based typing method reliant upon group-specific amplification to achieve an unambiguous second-field DQA1 typing assignment is presented. Method validation, using 51 reference DNA samples covering 21 different DQA1 alleles, showed 100% concordance with the reference types. This typing strategy has several important uses including identifying DQA1 mismatches in kidney donor/recipient pairs to inform patient DQ antibody assignments.

