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Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

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Published on: August 24, 2021

High-throughput methods for screening liposome-macrophage cell interaction.

Ciara Kelly¹, Ciaran Lawlor¹, Colin Burke¹

¹a School of Pharmacy, Royal College of Surgeons in Ireland , Dublin , Ireland.

Journal of Liposome Research

|December 31, 2014

Summary

This summary is machine-generated.

High-throughput screening of liposomes for drug delivery identified concentration-dependent efficacy. Mannose-modified liposomes showed efficacy dependent on mannosylated cholesterol linker length, aiding safe drug product development.

Keywords:

High-content analysis high-content screening liposomes nanoparticles nanotoxicology

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Area of Science:

Biomaterials Science
Drug Delivery Systems
Nanotechnology

Background:

Liposomes and lipid-based carriers are crucial for drug delivery, enhancing biocompatibility, efficacy, and targeting.
While drug discovery utilizes high-throughput screening, carrier material screening remains less explored.
Assessing carrier-cell interactions is vital for developing safe and effective biomedicines.

Purpose of the Study:

To adapt high-throughput methods for screening liposome-cell interactions.
To evaluate the cell interaction, toxicity, and immune reactivity of various liposomes.
To investigate the impact of liposome charge and mannose modification on these properties.

Main Methods:

Screening of neutral, anionic, cationic, and mannosylated liposomes.
Utilizing THP-1-derived macrophages for cell interaction assays.
Employing a high-throughput format for comprehensive analysis.

Main Results:

Liposome efficacy was observed to be concentration-dependent.
Mannosylated liposomes demonstrated efficacy that varied with mannosylated cholesterol linker length.
The study provides a high-throughput approach for evaluating liposome performance.

Conclusions:

High-throughput screening is effective for evaluating liposome-cell interactions.
Liposome characteristics, including charge and surface modification, significantly influence their performance.
This approach facilitates the development of safer and more efficient drug delivery systems.