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Claudin 1 mediates tumor necrosis factor alpha-induced cell migration in human gastric cancer cells

Atsushi Shiozaki1, Hiroki Shimizu1, Daisuke Ichikawa1

  • 1Atsushi Shiozaki, Hiroki Shimizu, Daisuke Ichikawa, Hirotaka Konishi, Shuhei Komatsu, Takeshi Kubota, Hitoshi Fujiwara, Kazuma Okamoto, Daisuke Iitaka, Shingo Nakashima, Yoshito Nako, Eigo Otsuji, Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.

World Journal of Gastroenterology
|December 31, 2014
PubMed
Abstract

Insights

Claudin 1 knockdown inhibits gastric cancer cell migration and invasion. It also significantly impacts tumor necrosis factor alpha (TNF-α)-induced gene expression, suggesting claudin 1 is a key regulator in gastric cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Gastric adenocarcinoma is a significant global health concern.
  • Cell migration and invasion are critical processes in cancer metastasis.
  • Tumor necrosis factor alpha (TNF-α) plays a complex role in cancer development.

Purpose of the Study:

  • To investigate the role of claudin 1 in regulating genes involved in cell migration.
  • To determine claudin 1's function in TNF-α-induced gene expression in gastric cancer cells.

Main Methods:

  • Claudin 1 was knocked down using small interfering RNA (siRNA) in MKN28 gastric adenocarcinoma cells.
  • Cell proliferation, apoptosis, migration, and invasion were analyzed.
  • Gene expression profiles were assessed using microarray and bioinformatics.

Main Results:

  • Claudin 1 knockdown significantly reduced cell proliferation, migration, and invasion, while increasing apoptosis.
  • Microarray analysis revealed 245 genes affected by claudin 1 knockdown, with cellular movement pathways being prominent.
  • Claudin 1 depletion inhibited 80% of TNF-α-induced mRNA expression changes and blocked TNF-α-enhanced cell migration.

Conclusions:

  • Claudin 1 acts as a crucial mediator in TNF-α-induced gene expression and cell migration in gastric cancer.
  • Understanding these mechanisms could lead to novel therapeutic strategies for gastric cancer.
Keywords:
Cell migrationClaudin 1Gene expression changeMicroarrayTumor necrosis factor alpha

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