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Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Related Experiment Video

Updated: Apr 19, 2026

Precision of In Vivo Quantitative Tooth Wear Measurement Using Intra-Oral Scans

Precision of In Vivo Quantitative Tooth Wear Measurement Using Intra-Oral Scans

Published on: July 12, 2022

Dental wear: a scanning electron microscope study.

Luca Levrini¹, Giulia Di Benedetto¹, Mario Raspanti¹

¹Department of Surgical and Morphological Sciences, Oro Cranio Facial Disease and Medicine Research Centre, Insubria University, 21100 Varese, Italy.

Biomed Research International

|December 31, 2014

Summary

This summary is machine-generated.

This study used scanning electron microscopy (SEM) to analyze four types of dental wear: erosion, attrition, abrasion, and abfraction. Identifying specific wear morphologies is crucial for diagnosing causes and related health issues.

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Area of Science:

Dentistry
Oral Pathology
Biomaterials Science

Background:

Dental wear is classified based on morphology and causes.
Understanding these differences is key for diagnosis and identifying systemic links.

Purpose of the Study:

To differentiate dental wear types (erosion, attrition, abrasion, abfraction) using SEM.
To analyze macro- and micromorphological features of lesions.
To clarify clinical presentations and diagnostic significance.

Main Methods:

Examined twelve extracted human teeth with distinct wear types.
Utilized scanning electron microscopy (SEM) for detailed analysis.
Evaluated enamel, dentin, enamel prisms, dentinal tubules, and pulp structures.

Main Results:

Provided a comprehensive overview of distinctive lesion morphologies for each wear type.
Confirmed existing knowledge on dental wear characteristics.
Highlighted specific SEM findings for erosion, attrition, abrasion, and abfraction.

Conclusions:

Each dental wear type exhibits a unique morphology and etiological mechanism.
Accurate identification aids in recognizing contributing factors and potential non-dental disorders.
Further research is needed to elucidate the etiology of abfraction.