Related Experiment Video
Updated: Apr 19, 2026
10:02
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
Published on: May 27, 2021
4.6K
Magnetic apatite for structural insights on the plasma membrane
Sarmiza E Stanca1, Robert Müller, Jan Dellith
1Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany.
Nanotechnology
|December 31, 2014
Summary
New magnetic iron oxide-hydroxyapatite (FeOxHA) nanoparticles with carboxymethyldextran (CMD) interact with cell membranes. These unique nanoparticles show potential for magnetic cell sorting applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cell Biology
Background:
- Developing novel nanomaterials for biological applications is crucial.
- Iron oxide-hydroxyapatite (FeOxHA) nanoparticles offer unique properties.
- Previous FeOxHA nanoparticles lacked homogeneity and ease of preparation.
Purpose of the Study:
- To develop homogeneous and easily prepared FeOxHA nanoparticles.
- To investigate the interaction of these nanoparticles with cellular membranes.
- To explore their potential in cell sorting and differentiation.
Main Methods:
- Synthesis of iron oxide-hydroxyapatite (FeOxHA) nanoparticles with carboxymethyldextran (CMD).
- Characterization using Raman spectroscopy, scanning electron microscopy (SEM), and fluorescence microscopy.
- Assessment of nanoparticle interaction with cell membranes.
Main Results:
- FeOxHA nanoparticles demonstrated homogeneity and simple preparation.
- The nanoparticles adhere to the plasma membrane without penetration.
- Combined magnetic, Raman, and cell membrane interaction properties were confirmed.
Conclusions:
- FeOxHA nanoparticles with CMD are unique for cell membrane interaction.
- These magnetic nanoparticles are promising for structural cell membrane differentiation.
- Potential applications include magnetic cell sorting in microfluidic devices.
More Related Videos
14:55Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
16.2K
05:56Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
3.4K
Related Concept Videos
01:25Applications Of NMR In Biology
4.8K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.8K
01:33Proteomics
10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.2K
01:07Nuclear Magnetic Resonance (NMR): Overview
8.6K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
8.6K
01:19Fluid Mosaic Model
20.4K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
20.4K
01:24Magnetic Resonance Imaging
10.5K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
10.5K
01:05Atomic Nuclei: Magnetic Resonance
1.4K
The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
1.4K