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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Updated: Apr 19, 2026

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Modification of a single-molecule AFM probe with highly defined surface functionality.

Fei Long¹, Bin Cao², Ashok Khanal²

¹Department of Mechanical Engineering-Engineering Mechanics, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan, USA.

Beilstein Journal of Nanotechnology

|January 1, 2015

Summary

This summary is machine-generated.

This study presents an easy method for single-molecule probe modification using click chemistry. This technique allows for reproducible control of probe functionality at the single-molecule level for atomic force microscopy applications.

Keywords:

atomic force microscopy click reaction force spectroscopy single molecule modification

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Published on: July 10, 2019

Area of Science:

Biophysics
Surface Chemistry
Nanotechnology

Background:

Single-molecule force spectroscopy (SMFS) using atomic force microscopy (AFM) is crucial for studying molecular interactions.
Achieving single-molecule resolution in AFM requires precise control over probe functionalization.
Current methods for probe modification can be complex and lack reproducibility.

Purpose of the Study:

To develop an easy and reproducible method for single-molecule probe modification.
To enable controlled functionalization of AFM probes down to the single-molecule level.
To enhance the reliability of data obtained from SMFS experiments.

Main Methods:

Utilized the copper-catalyzed alkyne-azide cycloaddition ('click') reaction for probe modification.
Covalently attached excess terminal alkynes to the AFM probe apex.
Employed a bi-functional molecule with an azide and a carboxylic acid for controlled carboxylation.

Main Results:

Demonstrated a facile method for modifying AFM probes with high precision.
Achieved controlled carboxylation on the probe apex, enabling single-molecule functionality.
Exhibited high reproducibility in probe modification, crucial for consistent SMFS data.

Conclusions:

The developed click chemistry-based method offers a straightforward approach for single-molecule probe functionalization.
This technique significantly improves the definition and reproducibility of probe functionality in AFM.
The method facilitates reliable single-molecule force spectroscopy studies by ensuring precise control over molecular interactions.