Ty1 transposition in Saccharomyces cerevisiae is nonrandom
G Natsoulis1, W Thomas, M C Roghmann
1Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Genetics
|October 1, 1989
Summary
Researchers studied Ty1 transposition in yeast genes, finding insertion hotspots within transcribed regions. Ty1 elements showed a gradient of insertion frequency, with higher rates at the 5' end of URA3 and LYS2 loci.
Area of Science:
- Molecular Biology
- Genetics
- Yeast Biology
Background:
- Ty1 elements are retrotransposons in yeast.
- Understanding Ty1 insertion patterns is crucial for studying genome stability and gene regulation.
- Previous studies have identified non-random Ty1 insertion preferences.
Purpose of the Study:
- To investigate the insertion site preferences of Ty1 elements in the URA3 and LYS2 genes.
- To identify potential hotspots for Ty1 transposition.
- To analyze sequence features associated with Ty1 insertion sites.
Main Methods:
- Generating a large collection of Ty1 insertions using a GAL1-Ty1 fusion.
- Sequencing insertion sites of Ty1 elements in URA3 and LYS2 loci.
- Analyzing insertion frequency gradients and identifying hotspots.
Main Results:
- A gradient of Ty1 insertion frequency was observed, highest at the 5' end and lowest at the 3' end of both URA3 and LYS2 loci.
- Specific hotspots for Ty1 transposition were identified in both loci.
- All identified hotspots were located within the transcribed regions of the genes.
- Sequence analysis revealed only weak similarities at insertion sites.
Conclusions:
- Ty1 transposition exhibits non-random insertion patterns with a directional bias and distinct hotspots.
- Transcribed regions of genes are preferred targets for Ty1 insertion.
- The sequence determinants for Ty1 insertion remain largely elusive despite identified hotspots.
More Related Videos
10:08Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
09:12Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
Published on: February 27, 2026
Related Concept Videos
02:13Overview of Transposition and Recombination
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
02:57DNA-only Transposons
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
03:18Non-LTR Retrotransposons
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
01:27Glucose Transporters
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
01:24Transposons
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...