Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

3.6K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
3.6K
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

30.0K
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a...
30.0K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.8K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.8K
IR Spectrometers01:25

IR Spectrometers

3.6K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
3.6K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

9.3K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is...
9.3K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.8K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An optimized ultrafast transient absorption experiment from the visible to the near-infrared with the B matrix referencing technique.

The Review of scientific instruments·2026
Same author

Subtype-specific bone mineralization defects and early treatment amelioration in murine models of autosomal recessive osteopetrosis revealed by Raman spectroscopy.

Bone·2026
Same author

Hydroxy-Group Topology as a Molecular Trigger Between Antioxidant and Photosensitizing Properties in Dihydroxynaphthalenes.

ACS omega·2026
Same author

Nonequilibrium dynamics of high energy transitions in monolayer WSe<sub>2</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same author

Ultrafast Excited-State Dynamics of Dithienyltetrazine-Based Donor-Acceptor Copolymers.

The journal of physical chemistry. B·2026
Same author

Integration of 2D Materials in Radial van der Waals Heterostructure Metasurfaces.

ACS nano·2026
Same journal

Cluster assisted soft-landing hub (CLASH): An instrument for surface desorption and deposition using a pulsed cluster ion source.

The Review of scientific instruments·2026
Same journal

Influence of pre-ionization parameters on multi-channel discharge characteristics of field-distortion switch gaps.

The Review of scientific instruments·2026
Same journal

A Joule-Thomson low-temperature scanning tunneling microscope with vector magnet and rotatable scanning head.

The Review of scientific instruments·2026
Same journal

Fiber-optic triggering of a two-stage high-current linear transformer driver with laser energy below 100 μJ.

The Review of scientific instruments·2026
Same journal

Optimization of laboratory-scale x-ray absorption spectroscopy (XAS) apparatus for nuclear fuel research.

The Review of scientific instruments·2026
Same journal

Compressed multi-scale entropy and its application in mechanical fault diagnosis.

The Review of scientific instruments·2026
See all related articles

Related Experiment Video

Updated: Apr 19, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

802

Two-dimensional electronic spectroscopy with birefringent wedges.

Julien Réhault1, Margherita Maiuri1, Aurelio Oriana1

  • 1IFN-CNR, Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.

The Review of Scientific Instruments
|January 3, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed a simple, stable experimental setup for rapid two-dimensional (2D) electronic spectroscopy using birefringent wedges. This technique offers broad spectral range and easy reconfiguration for studying complex molecular dynamics.

More Related Videos

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

2.2K
Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

9.3K

Related Experiment Videos

Last Updated: Apr 19, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

802
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

2.2K
Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

9.3K

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Ultrafast Science

Background:

  • Two-dimensional (2D) electronic spectroscopy is a powerful technique for probing ultrafast molecular dynamics.
  • Existing setups can be complex, requiring active stabilization and limiting rapid data acquisition.

Purpose of the Study:

  • To present a simple, highly stable experimental setup for 2D electronic spectroscopy.
  • To enable rapid data acquisition and broad spectral applicability.

Main Methods:

  • Utilizing a sequence of birefringent wedges to generate and delay phase-locked, collinear pump pulses.
  • Implementing continuous delay scanning without active stabilization.
  • Introducing a simple method for scattering suppression.

Main Results:

  • Achieved high phase stability and reproducibility in the collinear pump-probe setup.
  • Demonstrated rapid and easy recording of 2D spectra over a broad spectral range (UV to near-IR).
  • Successfully recorded degenerate and two-color 2D spectra of light-harvesting complex 1.

Conclusions:

  • The presented setup offers a user-friendly and robust platform for 2D electronic spectroscopy.
  • The technique's versatility supports studies across various spectral ranges and pulse durations.
  • This method facilitates efficient investigation of complex molecular systems.