Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

2.1K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
2.1K
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

1.4K
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
1.4K
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

3.3K
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
3.3K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

4.4K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
4.4K
Nonideal Two-Component Liquid Solutions01:29

Nonideal Two-Component Liquid Solutions

117
Nonideal liquid solutions, also known as real solutions, do not strictly follow Raoult's law. Raoult's law is a rule of thumb in physical chemistry. However, not all mixtures adhere to this law due to varying molecular interactions. For example, in an acetone/chloroform solution, the individual vapor pressures of the components are lower than expected, resulting in a total vapor pressure below that predicted by Raoult's law, causing a negative deviation.On the other hand, in an ethanol/water...
117
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

2.8K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lévy Formulation of the Stochastic Theory of Chromatography and Extension to Phase-Type Markov Renewal Process.

Analytical chemistry·2026
Same author

Addressing method transfer and robustness challenges using design space modeling.

Journal of chromatography. A·2026
Same author

Phase-Specific Parameter Estimation in Chiral HPLC Using 1-Site, 2-Site Stochastic Models, and Unified Equation Approach.

Analytical chemistry·2026
Same author

Ketogenic Diet and Brain Health: Cerebrovascular Mechanisms, Neuroprotection, and Translational Implications.

Nutrients·2026
Same author

Undiagnosed Diabetes in Metabolically Unhealthy Normal Weight Adults: A Cross-Sectional Analysis of National Health and Nutrition Examination Survey Cycle 2017-2020 in the United States.

Journal of clinical medicine·2026
Same author

Sequential model predictive direct speed control of PMSM.

Scientific reports·2026

Related Experiment Video

Updated: Apr 19, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

7.0K

Solvent minimization in two-dimensional liquid chromatography.

Krisztián Horváth1, Annamária Sepsey2, Péter Hajós1

  • 1Department of Analytical Chemistry, University of Pannonia, Egyetem utca 10, H-8200 Veszprém, Hungary.

Journal of Chromatography. A
|January 4, 2015
PubMed
Summary

This study presents an algorithm to minimize organic solvent use in comprehensive two-dimensional liquid chromatography (2DLC). Optimizing fraction collection and using efficient columns significantly reduces eluent consumption and environmental impact.

Keywords:
Eluent consumptionGreen chromatographyOptimizationSeparation powerSolvent reductionTwo-dimensional liquid chromatography

More Related Videos

Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector
08:54

Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector

Published on: May 30, 2025

1.6K
Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
07:50

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Published on: December 8, 2023

1.3K

Related Experiment Videos

Last Updated: Apr 19, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

7.0K
Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector
08:54

Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector

Published on: May 30, 2025

1.6K
Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
07:50

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Published on: December 8, 2023

1.3K

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Comprehensive two-dimensional liquid chromatography (2DLC) offers high peak capacity.
  • High peak capacity often correlates with increased eluent consumption, raising cost and environmental concerns.

Purpose of the Study:

  • To develop an algorithm for minimizing organic solvent consumption in 2DLC.
  • To investigate the relationship between peak capacity, eluent usage, and operational parameters in 2DLC.

Main Methods:

  • Development of a novel algorithm for solvent minimization in 2DLC.
  • Computational analysis of eluent consumption based on column dimensions and fraction analysis.
  • Derivation of an equation to optimize fraction collection for reduced solvent usage.

Main Results:

  • Higher peak capacities in 2DLC inherently require more eluent.
  • The equilibration volume of the second dimension has a minor impact on overall solvent consumption.
  • Optimizing the number of collected fractions significantly impacts eluent usage, allowing for reduced consumption without sacrificing peak capacity.

Conclusions:

  • 2D separations can be optimized for eluent consumption.
  • Minimizing eluent usage in 2DLC necessitates the use of small, high-efficiency columns in the second dimension.
  • A derived equation aids in calculating optimal fraction collection to minimize solvent usage, cost, and environmental impact.