Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells: native ribonucleoprotein
Joseph A Cozzitorto1, Masaya Jimbo, Saswati Chand
1Department of Surgery, The Jefferson Pancreas, Biliary, and Related Cancer Center, Thomas Jefferson University, 623 College Building, 1015 Walnut St., Philadelphia, PA, 19107, USA.
Abstract:
Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis-acting regulatory regions in the mRNA transcript that mediate direct interactions with trans-acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.
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