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Updated: Apr 19, 2026

Viral Nanoparticles for In vivo Tumor Imaging
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Specific visualization of tumor cells using upconversion nanophosphors.

E A Grebenik1, A N Generalova1, A V Nechaev2

  • 1Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

Acta Naturae
|January 6, 2015
PubMed
Summary

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Researchers developed a targeted nanoparticle construct for selective cancer cell labeling. This novel approach utilizes upconversion nanophosphors (UCNPs) and a HER2-targeting antibody fragment for improved medical diagnostics.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Biology

Background:

  • Targeted constructs using photoluminescent nanoparticles are crucial for medical diagnostics.
  • Upconversion nanophosphors (UCNPs) offer stable optical properties within biological tissues.
  • HER2 receptor overexpression is common in several human cancers, making it a key diagnostic target.

Purpose of the Study:

  • To develop a targeted two-component construct for selective labeling of HER2-overexpressing tumor cells.
  • To utilize upconversion nanophosphors (UCNPs) and an anti-tumor antibody fragment for enhanced tumor cell visualization.
  • To demonstrate the construct's efficacy in targeting specific cancer cell lines.

Main Methods:

  • Development of a two-component construct using UCNPs and 4D5 single-chain variable fragment (scFv).
Keywords:
HER2anti-tumor antibodiesbiomarker imagingself-assemblyupconversion nanophosphors

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  • Employment of a barnase:barstar (Bn:Bs) protein pair as a molecular adapter for self-assembly.
  • Testing the construct's binding selectivity against HER2-overexpressing SK-BR-3 cells.

    • Main Results:

    • A stable, targeted construct (4D5 scFv-Bn : UCNP-Bs) was successfully synthesized.
    • The construct demonstrated high affinity and selective binding to HER2-positive SK-BR-3 human breast adenocarcinoma cells.
    • The self-assembly mechanism using the Bn:Bs pair proved effective for construct formation.

    Conclusions:

    • The developed construct enables precise visualization of tumor cells overexpressing HER2.
    • This approach offers a versatile platform for creating similar constructs for detecting various disease markers.
    • The study highlights the potential of UCNP-based targeted therapies in oncology.