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Digital data acquisition and processing.

Mark A Naivar1, David W Galbraith2

  • 1DarklingX, LLC, Flow Cytometry, Los Alamos, New Mexico.

Current Protocols in Cytometry
|January 7, 2015
PubMed
Summary
This summary is machine-generated.

This guide details the data acquisition system in flow cytometers, explaining how electrical signals become cell data. It covers both analog and digital systems, focusing on modern digital data systems.

Keywords:
ADCDSPdata systemelectronicsprocessingpulsesignal

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Area of Science:

  • Biotechnology
  • Instrumentation
  • Data Science

Background:

  • Flow cytometers analyze individual cells using optical properties.
  • The data acquisition system converts detector signals into list-mode data.
  • Understanding this subsystem is key to instrument function and data interpretation.

Purpose of the Study:

  • To elucidate the inner workings of the flow cytometer data acquisition system.
  • To provide insights into the overall functioning of flow cytometers.
  • To aid in the assembly and understanding of data systems for practical use.

Main Methods:

  • Description of analog and digital data systems.
  • Focus on prevalent digital data systems in modern flow cytometry.
  • Explanation of signal conversion from optical detectors to list-mode data.

Main Results:

  • Detailed explanation of the data system's role in flow cytometry.
  • Demonstration of how electrical signals are transformed into usable data.
  • Insights into assembling and utilizing specific data systems.

Conclusions:

  • The data acquisition system is crucial for flow cytometer operation.
  • Modern flow cytometry relies heavily on digital data systems.
  • Advancements in electronics enable cost-effective data system construction.