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Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
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Poly(A)(+) mRNA-binding protein Tudor-SN regulates stress granules aggregation dynamics
Xingjie Gao1, Xue Fu, Juan Song
1Department of Biochemistry and Molecular Biology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, China; Laboratory of Molecular Immunology, Research Center of Basic Medical Science, Tianjin Medical University, China; Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin Medical University, China; Key Laboratory of Educational Ministry of China, Tianjin Medical University, China.
The FEBS Journal
|January 7, 2015
Summary
Tudor staphylococcal nuclease (Tudor-SN) is an SG-specific protein that regulates stress granule dynamics and stabilizes mRNA during cellular stress, clarifying its molecular mechanism.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Stress granules (SGs) and processing bodies (PBs) are cytoplasmic RNA foci formed during cellular stress.
- Previous studies suggested human Tudor staphylococcal nuclease (Tudor-SN) knockdown affects SG aggregation, but the mechanism was unclear.
Purpose of the Study:
- To elucidate the precise molecular mechanism of Tudor-SN in stress granule formation and function.
- To confirm the SG-specific localization of Tudor-SN.
Main Methods:
- Immunofluorescence microscopy to assess colocalization of Tudor-SN with SG/PB markers.
- Western blotting and immunoprecipitation to confirm protein interactions.
- RNA immunoprecipitation to identify associated mRNAs.
Main Results:
- Tudor-SN colocalizes with core SG components like poly(A)(+) mRNA binding protein 1, T-cell internal antigen-1-related protein, and poly(A)(+) mRNA.
- Tudor-SN interacts with SG/PB sharing proteins Argonaute 1/2 but not PB core proteins (e.g., decapping enzyme 1 a/b), confirming its SG specificity.
- Tudor-SN granules communicate with nuclear and cytosolic pools under stress.
- Tudor-SN regulates SG aggregation dynamics and selectively stabilizes SG-associated mRNA during stress.
Conclusions:
- Tudor-SN is an SG-specific protein that plays a crucial role in regulating SG dynamics.
- Tudor-SN actively participates in mRNA management within SGs during cellular stress, contributing to mRNA stabilization.
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