Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tyrosine monomer nanocrystal as a potent ice recrystallization inhibition activity.

Journal of materials chemistry. B·2026

Same author

Membrane-targeted DNA frameworks with biodegradability recover cellular function and morphology from frozen cells.

Trends in biotechnology·2025

Same author

Qualitative analysis of edible oil mixture for omega-3 content using terahertz time-domain spectroscopy.

NPJ science of food·2025

Same author

Photoinduced Plasmon Electron Transfer-based Bioorthogonal Cleavage Reaction for Precision Tumor Therapy.

Advanced materials (Deerfield Beach, Fla.)·2025

Same author

Engineering Bacterial Secretion Systems for Enhanced Tumor Imaging and Surgical Guidance.

Advanced materials (Deerfield Beach, Fla.)·2025

Same author

Plasmonic Observation of High-Density Nanoclustering in Low-Temperature H<sub>2</sub>O.

Small science·2025

Search research articles

Related Experiment Video

Updated: Apr 18, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

A "turn-on" fluorescent microbead sensor for detecting nitric oxide.

Lan-Hee Yang¹, Dong June Ahn², Eunhae Koo³

¹Advanced Materials Convergence Division, Korea Institute of Ceramic Engineering and Technology, Seoul, Republic of Korea ; Department of Biomicrosystem Technology, Korea University, Seoul, Republic of Korea.

International Journal of Nanomedicine

|January 8, 2015

Summary

This summary is machine-generated.

Researchers developed a novel fluorescent probe using microbeads to detect nitric oxide (NO) in vivo. This sensitive NO detection method is crucial for advancing treatments for cardiovascular diseases, cancers, and neurological disorders.

Keywords:

ab initio molecular simulation fluorescence microbead nitric oxide rhodium complex

More Related Videos

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Related Experiment Videos

Last Updated: Apr 18, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Area of Science:

Biomedical Engineering
Chemical Sensing
Molecular Imaging

Background:

Nitric oxide (NO) is a vital messenger molecule in biological systems, implicated in various physiological and pathological processes.
Accurate in vivo detection of NO is critical for diagnosing and treating diseases like cardiovascular conditions, cancers, and neurological dysfunctions.

Purpose of the Study:

To develop a highly sensitive fluorescent probe for detecting nitric oxide (NO) in biological systems.
To enhance fluorescence signal detection using microbead technology.

Main Methods:

Fabrication of microbeads infused with dansyl-piperazine (Ds-pip) fluorophore, coordinated with a rhodium (Rh)-complex.
Utilizing an "on/off" fluorescence mechanism triggered by the displacement of the Rh-complex by NO.
Employing Fritz Haber Institute ab initio molecular simulations (FHI-AIMS) for structural and binding energy calculations.

Main Results:

The developed probe exhibits fluorescence when NO replaces the Rh-complex, indicating successful NO detection.
Binding energy calculations confirm strong and rapid binding of NO to the Rh-core, displacing Ds-pip.
The simulations revealed an energy barrier for the recovery process, explaining its slower kinetics compared to quenching.

Conclusions:

The microbead-based fluorescent probe offers a sensitive method for in vivo nitric oxide detection.
The "on/off" mechanism is confirmed through molecular simulations, highlighting the probe's responsiveness to NO.
This technology holds promise for advancing diagnostics and therapeutics in NO-related diseases.