Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-specific RNA binding by the HIV-1 Rev protein.

M L Zapp1, M R Green

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Nature
|December 7, 1989
PubMed
Summary

The human immunodeficiency virus type 1 (HIV-1) Rev protein binds specifically to the Rev responsive element (RRE) on viral RNA. This binding is key to Rev

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improvements in Survival and Clinical Benefit With Gemcitabine as First-Line Therapy for Patients With Advanced Pancreas Cancer: A Randomized Trial.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2023
Same author

Matrix-assisted laser desorption/ionization-collision induced dissociation of poly(styrene).

Journal of the American Society for Mass Spectrometry·2016
Same author

Mutation of chromatin modifiers; an emerging hallmark of germinal center B-cell lymphomas.

Blood cancer journal·2015
Same author

BSACI guideline for the diagnosis and management of cow's milk allergy.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2014
Same author

The importance of the abdominal X-radiograph in acute ulcerative colitis.

Archives of disease in childhood. Education and practice edition·2014
Same author

Peptide sequence determination by matrix-assisted laser desorption ionization employing a tandem double focusing magnetic-Orthogonal acceleration time-of-flight mass spectrometer.

Journal of the American Society for Mass Spectrometry·2013

Area of Science:

  • Molecular Biology
  • Virology
  • Biochemistry

Background:

  • Human immunodeficiency virus type 1 (HIV-1) replication involves post-transcriptional regulation by the Rev protein.
  • Rev increases cytoplasmic levels of viral gag-pol and env messenger RNAs (mRNAs).
  • The precise mechanism of Rev action, potentially involving nuclear export or splicing modulation, remains unclear.

Purpose of the Study:

  • To elucidate the mechanism of HIV-1 Rev protein action.
  • To identify the specific binding interaction of Rev with its target mRNAs.

Main Methods:

  • Investigated the sequence-specific binding of the Rev protein.
  • Identified the Rev responsive element (RRE) as the binding site for Rev on viral mRNAs.

Main Results:

  • Demonstrated that the HIV-1 Rev protein is a sequence-specific DNA-binding protein.
  • Confirmed that the Rev responsive element (RRE) is the direct binding site for the Rev protein on viral RNA.

Conclusions:

  • Rev's interaction with the RRE is crucial for its function in regulating viral gene expression.
  • This finding clarifies the mechanism of Rev action, highlighting its role as a sequence-specific RNA-binding protein.

Related Experiment Videos