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Regulation of growth of human bladder cancer by miR-192
Yongchao Jin1, Jiasun Lu, Jiling Wen
1Department of Urology, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai, 200120, China.
Abstract:
The regulation of microRNA-192 (miR-192) is impaired in many cancers. Here, we investigated the role of miR-192 in the proliferation, cell cycle progression, and apoptosis of bladder cancer cells. Human bladder cancer cells were transfected with human miR-192 precursor or non-specific control miRNA. The effect of miR-192 on cell proliferation was assessed by a MTT assay. The effects of miR-192 on cell cycle regulation and apoptosis were evaluated by flow cytometry. Western blot was used to analyze the protein levels of cyclin D1, p21, p27, Bcl-2, Bax, and Mcl-1. We found that overexpression of miR-192 significantly decreased the proliferation of bladder cancer cells by 22 and 54 % at 48 and 72 h, respectively. MiR-192-overexpressing cells exhibited a significant increase in G0/G1 phase and a significant decrease in S phase compared to the control miRNA-transfected cells. Moreover, overexpression of miR-192 significantly induced apoptotic death in bladder cancer cells, increased the levels of p21, p27, and Bax, and decreased the levels of cyclin D1, Bcl-2, and Mcl-1. Taken together, these data suggest that miR-192 may be a suppressor for bladder cancer cells by cell cycle regulation.
Insights
MicroRNA-192 (miR-192) acts as a tumor suppressor in bladder cancer. Overexpressing miR-192 inhibits cancer cell proliferation and induces apoptosis by regulating the cell cycle.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNA-192 (miR-192) dysregulation is implicated in various cancers.
- Understanding miR-192's role in bladder cancer is crucial for therapeutic development.
Purpose of the Study:
- To investigate the function of miR-192 in bladder cancer cell proliferation, cell cycle progression, and apoptosis.
- To elucidate the molecular mechanisms underlying miR-192's effects in bladder cancer.
Main Methods:
- Human bladder cancer cells were transfected with miR-192 precursor or control miRNA.
- Cell proliferation was assessed using MTT assays.
- Cell cycle distribution and apoptosis were analyzed by flow cytometry.
- Protein levels of key cell cycle and apoptosis regulators (cyclin D1, p21, p27, Bcl-2, Bax, Mcl-1) were determined via Western blot.
Main Results:
- Overexpression of miR-192 significantly reduced bladder cancer cell proliferation at 48 and 72 hours.
- MiR-192-overexpressing cells showed increased G0/G1 phase arrest and decreased S phase.
- Apoptosis was significantly induced by miR-192, with altered expression of apoptosis-related proteins (increased p21, p27, Bax; decreased cyclin D1, Bcl-2, Mcl-1).
Conclusions:
- miR-192 functions as a tumor suppressor in bladder cancer.
- miR-192 exerts its suppressive effects by regulating cell cycle progression and inducing apoptosis.
- These findings highlight miR-192 as a potential therapeutic target for bladder cancer.
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