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Updated: Apr 18, 2026
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
A click chemistry approach to site-specific immobilization of a small laccase enables efficient direct electron
Dongli Guan1, Yadagiri Kurra, Wenshe Liu
1Chemical Engineering Department, Texas A&M University, College Station, TX 77843, USA.
Abstract:
Controlled orientation of a small laccase on a multi-walled carbon nanotube electrode was achieved via copper-free click chemistry mediated immobilization. Modification of the enzyme was limited to only the tethering site and involved the genetic incorporation of the unnatural amino acid 4-azido-L-phenylalanine (AzF). This approach enabled efficient direct electron transfer.
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