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Updated: Apr 18, 2026

Visualization of Organelles In Situ by Cryo-STEM Tomography
Published on: June 23, 2023
Quantitative evaluation of annular bright-field phase images in STEM
Takafumi Ishida1, Tadahiro Kawasaki2, Takayoshi Tanji3
1Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8603, Japan takafu_i@nuee.nagoya-u.ac.jp.
Abstract:
A phase reconstruction method based on multiple scanning transmission electron microscope (STEM) images was evaluated quantitatively using image simulations. The simulation results indicated that the phase shift caused by a single atom was proportional to the 0.6th power of the atomic number Z. For a thin SrTiO3 [001] crystal, the reconstructed phase at each atomic column increased according to the specimen thickness. The STEM phase images can quantify the oxygen vacancy concentration if the thickness is less than several nanometers.
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